The human SMCC/Mediator complex has recently been shown to be equivalent to the earlier-described TRAP complex and, through subunit specific interactions, to mediate the function of a variety of activators (including several nuclear receptors, the tumor suppressor p53, and the herpes virus activator VP16). The Department of Biochemistry and Molecular Biology is an established basic science department that plays a major role in the research and education missions of Thomas Jefferson University. As molecular biology has become an integral part of almost all biological courses, the third section of this book is dedicated to all the techniques related to and used in molecular biology. Chongyi Chen investigates the mechanistic link between DNA topology, chromatin structure and gene expression, from a single-cell and genome-wide perspective. Discover how we're translating breakthroughs in the lab into clinical applications that transform lives. He co-developed the wheat-germ cell-free protein synthesis system, one of the first methods used to analyze functional mRNA levels in normal and transformed cells and tissues. Our studies are also directed toward the mechanisms by which these factors in turn are regulated in the various biological responses. The lab develops and employs cutting-edge technology in single-cell omics and imaging to study chromosome biology in mammalian systems. Read the latest chapters of Laboratory Techniques in Biochemistry and Molecular Biology at ScienceDirect.com, Elsevier’s leading platform of peer-reviewed scholarly literature His research is focused on determining high-resolution structures of protein folding intermediates, histone variants in complex with their chaperones, and nucleosomes in complex with nucleosome-binding proteins. About us. Our broad objectives are to understand the specific regulatory events that control these processes, as well as more fundamental aspects of transcription activation and repression mechanisms. Since the ubiquitous general transcription factors represent the ultimate targets of the various gene-specific factors, their characterization is essential. Biochemistry and Molecular Biology Laboratory Welcome. Find out more . Michael Lichten investigates mechanisms of DNA damage repair and homologous recombination, focusing on meiotic recombination in the budding yeast Saccharomyces cerevisiae. Yawen Bai investigates the mechanism of protein folding and chromatin dynamics using biophysical techniques, including nuclear magnetic resonance (NMR). The processes of animal cell growth, differentiation and infection by viruses result from the differential expression of specific genes, controlled primarily at the level of transcription. The premier Australian Department in its discipline, our research and teaching aims to understand and communicate biological processes and how these are altered in disease. Shiv Grewal investigates the mechanisms of heterochromatin assembly and their roles in maintaining genome stability using the fission yeast Schizosaccharomyces pombe as a model system. The laboratory is approximately 1500 square feet and is well-equipped, including a cell and tissue culture room, a microscopy room, and a large area with equipment to explore the cellular and biochemical responses … The roles of DNA repair, the DNA damage response, and cell cycle regulatory proteins in homologous recombination are also explored. He has been a major contributor to the study of myogenesis in both vertebrates and in Drosophila, and was one of the first to demonstrate RNAi knock-down to study gene function during Drosophila development. Biochemistry and Molecular Biology. Biochemistry and Molecular Biology Journal is an international scholarly, peer reviewed journal presenting original research contributions and scientific advances related to the field of Biochemistry & Molecular Biology. The general cofactors include both negative cofactors (NC2, TAFs) that repress (basal) promoter activity in the absence of activators and positive cofactors (PC1, PC2, PC3 and PC4) that, in the presence of activators, reverse the action of negative cofactors and effect a net increase in overall promoter activity. U.S. Department of Health and Human Services | National Institutes of Health | National Cancer Institute | USA.gov, Home | Contact | Policies | Accessibility | Viewing Files | FOIA, Laboratory of Biochemistry and Molecular Biology, U.S. Department of Health and Human Services.